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Head: Aleksandra Rusin, PhD | 
Head: Katarzyna Lisowska, PhD |
Head: Wiesława Widłak, PhD | 
Head: Marek Rusin, PhD |
HEAD: ALEKSANDRA RUSIN, PhD
STAFF:
Anna Zborek PhD,
Ewa Małusecka PhD,
Magdalena Głowala-Kosińska MSc,
Irena Peick,
Apolonia Kyrcz
RESEARCH PROFILE:
expression of selected molecular tumor markers,
expression and function of heat shock proteins,
expression of selected cell cycle related proteins,
cell cycle abnormalities and ploidy disorders
PRINCIPAL TECHNIQUES:
immunohistochemistry,
immunofluorescence,
microdissection,
flow cytometry,
cytotoxicity assays
CURRENT RESEARCH ACTIVITIES:
Diagnostic and prognostic molecular markers in prostate cancer
The main aim of a project is to determine the usefulness of molecular markers AMACR i p63 in diagnostics of difficult/conflicting cases of prostate cancer. Research on predictive markers for therapeutic response to radiotherapy in patients with prostate cancer are focused on bcl-2/bax ratio. Project is held in cooperation with Tumor Pathology Departament, II Radiotherapy Clinic/Teaching Hospital, Radiodiagnostics Department and Radiotherapy Department.
The influence of cytoprotective function of heat shock proteins on tumorigenesis and anticancer therapy
Stress-inducible protein HSP70i is frequently constitutively expressed at high levels in primary tumors. We are interested whether HSP70i can modify resistance of cancer cells to apoptosis induced by aneugenic drugs, and whether it can affect the frequency of mitotic spindle malformations.
Role of heat shock proteins in inflammation.
The aim of the study is to determine the expression pattern of HSPs (mainly HSP70n and HSP25) in rat liver in which inflammatory reaction is induced by administration of various hepatotoxicants. The most interesting for us is to study the mechanisms of HSP27 induction in hepatocytes during macrophages infiltration.
Novel genistein derivatives as radiosensitizers.
Flavonoids are known to enhance radiosensitivity due to inhibition of the cell cycle progression in the phase G2/M. New genistein derivatives, patented by Pharmaceutical Institute are much more effective G2/M blockers than genistein. The aim of a study is to test radiosensitizing properties of new genistein derivatives in prostate cancer cell lines. The project is realized in cooperation with Pharmaceutical Institute.
Application of laser-captured microdissection for gene expression profiling in thyroid cancer.
The project of gene expression profililing in medullary thyroid cancer is held in cooperation with Nuclear Medicine and Endocrine Oncology Department. Pure cell populations are isolated with laser microdissection technique. Compared to RNA from bulk material now even minute differences in expression become detectable by quantitative RT-PCR or cDNA-array hybridization methods.
INSTRUMENTATION:
Microdissector PALM OLYMPUS
Fully motorized inverted microscope Olympus IX 81 equiped with phase contrast, Nomarski DIC and fluorescence enables the identification of objects. Microdissection based on laser microdissection & pressure catapulting technology allows to isolate material from routine histological sections, cytospins or cell cultures for downstream biomolecular analysis of DNA, RNA or proteins. Pulsed ultra-violet (UV) laser of high beam quality interfaced into the microscope and focused through an objective to a beam spot size of less than 1 micrometer in diameter allows for micropreparation of single cells and subcellular particles.
Flow cytometer BD FACSCanto
The instrument is built with blue (488 nm, air-cooled, 20 mW solid state) and red (633 nm, 17 mW HeNe) excitation sources. The instrument allows for the simultanous analysis of different fluorochromes: APC, APC-Cy7, FITC, PE, PerCP or PerCP-Cy5.5, PE-Cy7. The system allows for the signal retention on six fluorescent and two scatter parameters and processing up to 10,000 events per second. It has a capacity for eight-parameter detection while capturing particle sizes from 0.5 to 50 ľm in diameter, enabeling projects with leucocytes, cell lines, platelets, multiplexed bead technologies. Multiparameter detection helps advanced cell subset analysis, multiparameter functional studies, soluble protein detection, and quantification studies for cell surface antigens and cellular proteins.
Research NIKON Eclipse E800 upright microscope
Research microscope equipped with image analysis software.
HEAD: KATARZYNA LISOWSKA PhD
STAFF:
dr Anna Fiszer-Kierzkowska,
lek. med. Michał Jarząb,
mgr Magdalena Olbryt,
mgr Volha Dudaladava,
Krystyna Klyszcz
RESEARCH PROFILE:
Our main scientific interests are concerned with the molecular mechanisms of neoplastic transformation of ovarian and breast epithelial cells.
PRINCIPAL TECHNIQUES/INSTRUMENTATION:
Main methods employed in our research are expression profiling by DNA microarrays (Affymetrix Gene Chip platform), different types of PCR (PCR-RFLP, ASA-PCR, Real-Time RT-PCR), molecular cloning, sequencing (Abi Prism 377) and other molecular biology methods.
CURRENT RESEARCH TOPICS ARE:
expression profiling in breast and ovarian cancer
studies of the molecular background underlying different clinical presentation of hereditary versus sporadic breast and ovarian cancers
search for the molecular markers suitable for diagnostics, prognosis and monitoring of the disease
studies of the molecular mechanism of differential sensitivity of breast and ovarian cancer to various schemes of chemotherapy
search for the genetic risk factors predisposing to breast and ovarian cancers; evaluation of the frequency of polymorphic variants of different genes possibly linked to the etiology of these malignancies, search for novel predisposing mutations and polymorphisms
HEAD: WIESŁAWA WIDŁAK PhD
STAFF:
Dorota Ścieglińska PhD,
Natallia Vydra PhD,
Agnieszka Mazurek MSc,
Wojciech Pigłowski MSc,
Alexei Mikhailov MSc.
RESEARCH PROFILE:
regulation of HSP70 (70kDa heat shock protein) genes expression in humans and rodents
role of HSP70 in cancer cells
differential expression and regulation of HSP70 genes in somatic and male germ cells
role of neuropilins in cancer cells migration.
PRINCIPAL TECHNIQUES:
transgenic mice,
cell culture techniques (transient and stable transfection, RNAi, biochemical and fluorescent reporter genes analysis, cell migration assays),
analysis of DNA, RNA and protein expression (Southern, Northern and Western blot, PCR, Real Time RT-PCR and RT-PCR-based methods),
DNA cloning (construction of hybrid genes, fusion proteins coding vectors).
CURRENT RESEARCH ACTIVITIES:
The molecular mechanisms of a cell type-specific function of Heat Shock Transcription Factor 1 (HSF1).
HSF1 is activated under stress conditions. In majority of somatic cells activation of HSF1 leads to synthesis of heat shock proteins, what is a part of cytoprotective response. Our results indicate that in differentiating male germ cells HSF1 induces receptor-mediated and mitochondria-mediated apoptosis that is not prevented by HSP70i. The main aim of our work is to identify genes that are differentially expressed in somatic versus spermatogenic cells upon activation of HSF1.
The function of HSP70 genes (mainly human HSP70i and HSPA2 genes) in cancer cells.
Our main interest is to determine the influence of inducible HSP70 and spermatocyte-specific HSPA2 proteins on the phenotype of human cancer cells and to estimate their cytoprotective and antiapoptotic properties.
Evaluation of biological significance of proteins involved in neuron migration for cancer cell behavior.
We intent to determine whether expression of NEUROPILIN-1 receptor on cancer cells surface is involved in cells migration.
INSTRUMENTATION:
Fully equipped laboratory of transgenic mice production by microinjection technique: Inverted microscope Axiovert 135 (Carl Zeiss) equipped with two manual micromanipulators enables microinjection of DNA into one-cell mouse embryos. Puller and microforge used for micropipette preparation, incubator used for cultivation of embryos.
Fully equipped cell culture laboratory: Dual Chamber Incubator model BB-6220 from Heraeus, laminar flow hoods for work in sterile condition.
HEAD: MAREK RUSIN PhD
STAFF:
Mieczysław Chorąży PhD, MD,
Dorota Butkiewicz PhD,
Małgorzata Krześniak MSc,
Rasa Vaitiekunaite MSc,
Iwona Matuszczyk
RESEARCH PROFILE:
molecular epidemiology of cancer (mainly lung cancer),
studies on RecQ helicase-containing mechanisms protecting stability of the genome,
cellular senescence as an antioncogenic mechanism,
functions of antioncogenic protein - PML.
PRINCIPAL TECHNIQUES:
polymerase chain reaction (PCR) used as a tool for genetic analysis and for creating recombinant DNA molecules;
site-directed mutagenesis and molecular cloning of DNA,
protein localization studies using fluorescent proteins,
functional analysis of gene promoters and transcription regulating proteins using reporter assays,
cell culture, searching for and identification of genetic mutations and polymorphisms using PCR-RFLP and DNA sequencing.
CURRENT RESEARCH ACTIVITIES:
Identification of genetic markers of lung cancer risk in women.
The rate of metabolic activation and detoxification of carcinogenic compounds as well as the DNA repair capacity are genetically determined host factors, which are able to modulate the cancer risk. We search for genetic markers, closely associated with functional alterations of cytoprotection mechanisms, which may be useful in determining the individual risk of cancer. We have the collection of DNA samples isolated from blood of female lung cancer patients and healthy controls. Using that DNA, we genotype the polymorphisms of genes coding for cytoprotection proteins. With this case-control approach (at least 200 lung cancer patients and 200 controls), we plan to analyze dozens of genetic markers (polymorphisms) using PCR-RFLP or DNA sequencing methods to determine their influence on cancer risk. Moreover, we search for germline p53 mutation in female lung cancer patients with strong family history of cancer.
Molecular and functional characterization of genes and proteins involved in maintenance of genomic stability and regulation of cellular senescence.
Maintenance of genomic stability protects against cancer and delays the appearance of aging. Cells contain many proteins involved in recombinational repair of DNA. Some of these proteins, e.g. RecQ helicases, also participate in maintenance of the telomere length. This research project involves: studies on mechanisms of gene expression regulation by PML - a protein regulating cellular senescence as well as the functioning of RecQ helicases. We search for protein domains of RecQ helicases that are responsible for intranuclear movement of the helicases in response to the genotoxic stress. We also analyze the influence of the RecQ helicases on the telomere structure and functioning.
INSTRUMENTATION:
ABI PRISM 377 DNA Sequencer. Equipment for cell culture.
RESEARCH PAPERS (2004-2007)
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- Jin Q., Hemminki K., Grzybowska E., Klaes R., Söderberg M., Försti A. (2004): Integrin b3 Leu33Pro homozygosity and risk of cancer. J. Natl. Cancer Inst. 96, 234-235
- Pack S.D., Alper O.M., Stromberg K., Augustus M., Ozdemirli M., Miermont A.M., Klus G., Rusin M., Slack R., Hacker N.F., Ried T., Szallasi Z., Alper O. (2004): Simultaneous suppression of epidermal growth factor receptor and c-erbB-2 reverses aneuploidy and malignant phenotype of a human ovarian carcinoma cell line. Cancer Res. 64, 789-794.
- Ścieglińska D., Vydra N., Krawczyk Z., Widłak W. (2004): Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene. Biochem. J. 379, 739-747.
- Rusin M., Zientek H., Krześniak M., Małusecka E., Zborek A., Krzyżowska-Gruca S., Butkiewicz D., Vaitiekunaite R., Lisowska K., Grzybowska E., Krawczyk Z. (2004): Intronic polymorphism (1541-1542delGT) of the constitutive heat shock protein 70 gene has functional significance and shows evidence of association with lung cancer risk. Mol. Carcinogen. 39, 155-163
- Försti A., Angelini S., Festa F., Sanyal S., Zhang Z., Grzybowska E., Pamuła J., Pękala W., Zientek H., Hemminki K., Kumar R. (2004): Single nucleotide polymorphisms in breast cancer. Oncol. Rep. 11, 917-922
- Widłak W., Widłak P. (2004): MAR/SAR elements flank the rat hst70 gene transcription unit. Cell. Mol. Biol. Lett. 9, 123-133
- Górski B., Jakubowska A., Huzarski T., Byrski T., Gronwald J., Grzybowska E. i wsp. (2004): A high proportion of founder BRCA1 mutations in polish breast cancer families. Int. J. Cancer 110, 683-686
- Piddubnyak V., Kurcok P., Matuszowicz A., Głowala M., Fiszer-Kierzkowska A., Jedliński Z., Juzwa M., Krawczyk Z. (2004): Oligo-3-hydroxybutyrates as potential carriers for drug delivery. Biomaterials 25, 5271-5279
- Krześniak M., Butkiewicz D., Samojedny A., Chorąży M., Rusin M. (2004): Polymorphisms in TDG and MGMT genes - epidemiological and functional study in lung cancer patients from Poland. Ann. Hum. Genet. 68, 300-312
- Jin Q., Hemminki K., Grzybowska E., Klaes R., Söderberg M., Zientek H., Rogozińska-Szczepka J., Utracka-Hutka B., Pamuła J., Pękala W., Försti A. (2004): Polymorphisms and haplotype structures in genes for transforming growth factor b1 and its receptors in familial and unselected breast cancers. Int. J. Cancer 112, 94-99
- Wagner K., Hemminki K., Grzybowska E., Klaes R., Butkiewicz D., Pamuła J., Pękala W., Zientek H., Mielżyńska D., Siwińska E., Försti A. (2004): The insulin-like growth factor-1 pathway mediator genes: SHC1 Met300Val shows a protective effect in breast cancer. Carcinogenesis 25, 2473-2478
- Rogozińska-Szczepka J., Utracka-Hutka B., Grzybowska E., Mąka B., Nowicka E., Smok-Ragankiewicz A., Zientek H., Steffen J., Wojciechowska-Łącka A. (2004): BRCA1 and BRCA2 mutations as prognostic factors in bilateral breat cancer patients. Ann. Oncol. 15, 1373-1376
- Łobocka M.B., Rose D.J., Plunkett G. III, Rusin M., Samojedny A., Lehnherr H., Yarmolinsky M.B., Blattner F.R. (2004): Genome of bacteriophage P1. J. Bacteriol. 186, 7032-7068.
- Smits K.M., Benhamou S., Garte S., Weijenberg M.P., Alamanos Y., Ambrosone C., Autrup H., Autrup J.L., Baranova H., Bathum L., Boffetta P., Bouchardy C., Brockmoller J., Butkiewicz D. i wsp. (2004): Association of metabolic gene polymorphisms with tobacco consumption in healthy controls, Int. J. Cancer 110, 266-270.
- Lisowska K., Grzybowska E. (2004): Profile ekspresji genów w raku jajnika - poszukiwanie nowych markerów molekularnych i próby klasyfikacji przypadków pod względem rokowania i odpowiedzi na terapię. Gin. Onkol., Supl. 1, 21-29.
- Popanda O., Schattenberg T., Phong C.T., Butkiewicz D., Risch A., Edler L., Kayser K., Dienemann H., Schulz V., Drings P., Bartsch H., Schmezer P. (2004): Specific combinations of DNA repair gene variants and increased risk for non-small cell lung cancer. Carcinogenesis 25, 2433-2441
- Butkiewicz D., Popanda O., Risch A., Edler L., Dienemann H., Schulz V., Kayser K., Drings P., Bartsch H., Schmezer P. (2004) Association between the risk for lung adenocarcinoma and a (-4) G-to-A polymorphism in the XPA gene. Cancer Epidemiol. Biomarkers Prev. 13, 2242-2246
- Lenartowicz M., Grzmil P., Rusin M., Styrna J. (2004) Alternative spilicing in the Atp7a gene in the Cu deficient mosaic mutation in mice. Folia biol. 52, 219-223
- Lisowska K., Jarząb M.: Zastosowania mikromacierzy w biologii molekularnej i naukach medycznych. W: Na Pograniczu Chemii i Biologii, red. H. Koroniak, J. Barciszewski, Uniwersytet im. Adama Mickiewicza w Poznaniu, Wyd. Nauk. Poznań 2004, t. X, str. 401-427
- Sengupta S., Shimamoto A., Koshiji M., Pedeux R., Rusin M., Spillare E.A., Shen J.C., Huang L.E., Lindor N.M., Furuichi Y., Harris C.C. Tumor suppressor p53 represses transcription of RECQ4 helicase. Oncogene 24,1738-1748
- Burwinkel B., Wirtenberger M., Klaes R., Schmutzler R.K., Grzybowska E., Forsti A., Frank B., Bermejo J.L., Bugert P., Wappenschmidt B., Butkiewicz D., Pamula J., Pekala W., Zientek H., Mielzynska D., Siwinska E., Bartram C.R., Hemminki K. (2005) Association of NCOA3 polymorphisms with breast cancer risk. Clin. Cancer Res. 11, 2169-2174
- Wilkening, S., B. Burwinkel, E. Grzybowska, R. Klaes, J. Pamula, W. Pekala, H. Zientek, K. Hemminki, A. Forsti (2005) Polyglutamine repeat length in the NCOA3 does not affect risk in familial breast cancer. Cancer Epidemiol. Biomarkers Prev. 14, 291-292
- Wagner,K., K. Hemminki, E. Israelson, E. Grzybowska, M. Soderberg, J. Pamuła, W. Pękala, H. Zientek, D. Mielżyńska, E. Siwińska, A. Forsti (2005) Polymorphisms in the IGF-1 and IGFBP3 promoter and the risk of breast cancer. Breast Cancer Res. Treat. 92, 133-140
- Jin, Q., K. Hemminki, K. Enquist, P. Lenner, E. Grzybowska, R. Klaes, R. Henriksson, B. Chen, J. Pamula, W. Pekala, H. Zientek, J. Rogozińska-Szczepka, B. Utracka-Hutka, G. Hallmans, A. Forsti (2005) Vascular endothelial growth factor polymorphisms in relation to breast cancer development and prognosis. Clin. Cancer Res. 11, 3647-3653
- Chorąży M. (2005) Geny i genetyka - nowe dylematy. Onkologia w Praktyce Klinicznej 1, 1-6.
- Debniak, T., B. Gorksi, T. Huzarski, T. Byrski, C. Cybulski, A. Mackiewicz, S. Cozdecka-Grodecka, J. Gronwald, E. Kowalska, O. Haus, E. Grzybowska, M. Stawicka, M. Swiec, K. Urbanski, S. Niepsuj, B. Wasko, S. Gozdz, P. Wandzel, C. Szczylik, D. Surdyka, A. Rozmiarek, O. Zambrano, M. Posmyk, S. Narod, J. Lubinski (2005) A Common Variant of CDKN2A (p16) Predisposes to Breast Cancer. J. Med. Genet. (przyjęta do druku).
- Wirtenderger M., Hemminki K., Forsti A., Klaes R., Schmutzler R.K., Grzybowska E., Bermejo J.L., Wappenschmidt B., Bugert P., Butkiewicz D., Pamuła J., Pękala W., Zientek H., Bartram C.R., Burwinkel B. (2005) c-MYC Asn11Ser is associated with increased risk for familial breast cancer. Int. J. Cancer, (przyjęta do druku).
- Raimondi S., Boffetta P., Anttila S., Brockmoller J., Butkiewicz D., Cascorbi I., Clapper M.L.,i wsp. (2005) Metabolic gene polymorphisms and lung cancer risk in non-smokers An update of the GSEC study. Mutat Res. (przyjęta do druku).
- Vydra N, Malusecka E, Jarzab M, Lisowska K, Glowala-Kosinska M, Benedyk K, Widlak P, Krawczyk Z, Widlak W. (2005). Spermatocyte-specific expression of constitutively active Heat Shock Factor 1 induces HSP70i-resistant apoptosis in male germ cells. Cell Death and Differentiation (przyjęta do druku).
- N. Vydra, E. Małusecka, M. Jarząb, K. Lisowska, M. Głowala-Kosińska, K. Benedyk, P. Widłak, Z. Krawczyk, W. Widłak (2006) Spermatocyte-specific expression of constitutively active heat shock factor 1 induces HSP70i-resistant apoptosis in male germ cells. Cell Death Differ. 13, 212-222.
- E. Małusecka, A. Zborek, S. Krzyżowska-Gruca, Z. Krawczyk (2006) Immunohisto-chemical detection of the inducible heat shock protein Hsp70. A methodological study. J. Histochem. Cytochem. 54, 183-190.
- J. Pamuła, M. Krześniak, H. Zientek, W. Pękala, M. Rusin, E. Grzybowska (2006) Functional impact of sequence alterations found in BRCA1 promoter/5’UTR region in breast/ovarian cancer families from Upper Silesia, Poland. Hereditary Cancer in Clinical Practice 4, 20-24.
- V. Dudaladava, M. Jarząb, E. Stobiecka, E. Chmielik, K. Simek, T. Huzarski, J. Lubiński, J. Pamuła, W. Pękala, E, Grzybowska, K. Lisowska (2006) Gene expression profiling in hereditary, BRCA1-linked breast cancer: preliminary report. Hereditary Cancer in Clinical Practice, 4, 28-38.
- G. Kramer-Marek, C. Serpa, A. Szurko, M. Wideł, A. Sochanik, M. Śnietura, P. Kus, R.M.D. Nunes L.G. Arnaut, A. Ratuszna (2006) Spectroscopic properties and photodynamic effects of new lipophilic porphyrin derivatives: Efficacy, localisation and cell death pathways. J. Photochem. Photobiol. B. 84, 1-14.
- K. Wagner, K. Hemminki, E. Grzybowska, J.L. Bermejo, D. Butkiewicz, J. Pamuła, W. Pękala, A. Försti (2006) Polymorpshisms in the growth hormone receptor: A case control study in breast cancer. Int. J. Cancer 118, 2903-2906.
- K. Wagner, K. Hemminki, E. Grzybowska, R. Klaes, B. Burwinkel, P. Bugert, R.K. Schmutzler, B. Wappenschmidt, D. Butkiewicz, J. Pamuła, W. Pękala, A. Försti (2006) Polymorphisms in genes involved in GH1 release and their association with breast cancer risk. Carcinogenesis 27, 1867-1875.
- A. Zborek, E. Malusecka, A. Rusin, S. Krzyżowska-Gruca, Z. Krawczyk (2006) Influx of macrophages into livers of rats treated with hepatotoxicants (thioacetamide, allyl alcohol, D: -galactosamine) induces expression of HSP25. J. Mol. Histol. 37, 381-389.
- M. Olbryt, M. Jarząb, J. Jazowiecka-Rakus, K. Simek, S. Szala, A. Sochanik (2006) Gene expression profile of B 16(F10) murine melanoma cells exposed to hypoxic conditions in vitro. Gene Expr. 13, 191-203.
- W. Rupik, A. Stawierej, I. Stolarczyk, W. Widłak (2006) Promoter of the heat shock testis-specific Hsp70.2/Hst70 gene is active in nervous system during embryonic development of mice. Anat. Embryol. 211, 631-638.
- Z. Wygoda, D. Kula, G. Bierzyńska-Macyszyn, D. Larysz, M. Jarząb, P. Właszczuk, P. Bażowski, M. Wojtacha, A. Rudnik, T. Stępień, W. Kasperka, A. Etmańska, K. Składowski, R. Tarnawski, D. Kokocińska, B. Jarząb (2006) Use of monoclonal anti EGFR antibody in the radioimmunotheraphy of malignant gliomas in the context of EGFR expression in grade III and IV tumors. Hybridoma 25, 125-132.
- D. Kula, T. Bednarczuk, B. Jurecka-Lubieniecka, J. Polańska, K. Hasse-Lazar, M. Jarząb, K. Steinhof-Radwańska, B. Hejduk, J. Żebracka, A. Kuryłowicz, E. Bar Andziak, T. Stęchły, A. Pawlaczek, E. Gubała, A. Krawczyk, S. Szpak-Ulczok, J. Nauman, B. Jarząb (2006) Interaction of HLA-DRB1 alleles with CTLA-4 in the predisposition to Grave’s disease: The impact of DRB1*07. Thyroid 16, 447-453.
- W. Widłak (2006) Odpowiedź na stres komórkowy i ekspresja genów Hsp70 w męskich komórkach rozrodczych. Postępy Biochemii 52, 289-295.
- O. Popanda, L. Edler, P. Waas, T. Schattenberg, D. Butkiewicz, T. Muley, H. Dienemann, A. Risch, H. Bartsch, P. Schmezer (2007) Elevated risk of squamous-cell carcinoma of the lung in heavy smokers carrying the variant alleles of the TP53 Arg72Pro and p21 Ser31Arg polymorphisms. Lung Cancer 55, 25-34.
- W. Widłak, B. Winiarski, A. Krawczyk, N. Vydra, E. Małusecka (2007) Inducible 70 kDa heat shock protein does not protect spermatogenic cells from damage induced by cryptorchidism. Int. J. Androl. 30, 80-87.
- W. Widłak, N. Vydra, E. Małusecka, V. Dudaladava, B. Winiarski, D. Ścieglińska, P. Widłak (2007) Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes Cells 12, 487-499.
- W. Pigłowski, R. Nowak, Z. Krawczyk, D. Ścieglińska (2007) The structural and functional analysis of the human HSPA2 gene promoter region. Acta Biochim. Polon. 54, 99-106.
- W. Widłak, N. Vydra, V. Dudaladava, D. Ścieglińska, B. Winiarski,Z. Krawczyk (2007) The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene. Acta Biochim. Polon. 54, 107-112.
- E. Nagy, Z. Balogi, I. Gombos, M. Åkerfelt, A. Björkbom, G. Balogh, Z. Török, A. Maslyanko, A. Fiszer-Kierzkowska, K. Lisowska, P. J. Slotte, L. Sistonen, I. Horváth, L. Vigh (2007) Hyperfluidization-coupled membrane microdomain reorganization is linked to activation of the heat shock response in a murine melanoma cell line. PNAS 104, 7945-7950.
- K. Wagner, E. Grzybowska, D. Butkiewicz, J. Pamuła-Pilat, W. Pękala, K. Tęcza, K. Hemminki, A. Försti (2007) High-throughput genotyping of a common deletion polymorphism disrupting the TRY6 gene and its association with breast cancer risk. BCM Genetics 8, 41.
- R. Vaitiekunaite, D. Butkiewicz, M. Krześniak, M. Przybyłek, A. Gryc, M. Śnietura, M. Benedyk, C.C. Harris, M. Rusin (2007) Expression and localization of Werner syndrome protein is modulated by SIRT1 and PML. Mech. Ageing Dev. 128, 650-651.
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GRANTS:
| Grant Nr 2 P05A 125 28 | (10.06.2005 - 31.12.2007) |
| "Zbadanie ekspresji helikaz z rodziny RecQ, BLM i WRN, uczestniczących w utrzymaniu tabilności genomu - związek z antyonkogennym białkiem PML", Kierownik projektu - Dr Dorota Butkiewicz |
| Grant Nr 2 P04A 040 30 | (01.06.2006 - 31.05.2009) |
| "Mechanizm indukcji apoptozy przez czynnik transkrypcyjny HSF1 (heat shock transcription factor 1)", Kierownik projektu - Dr Wiesława Widłak |
| Grant Nr PBZ-MNiL1/1/2005 | (09.11.2006 - 08.05.2009) |
| "Opracowanie nowego leku z grupy pochodnych izoflawonów o przewidywanym działaniu przeciwnowotworowym", Kierownik projektu - Prof. dr hab. Zdzisław Krawczyk |
| Grant Nr PBZ-MNiL2/1/2005 | (21.11.2006 - 02.11.2009) |
| "Znaczenie podstawowych molekularnych cech nowotworu oraz jego mikro - środowiska jako determinant profilu ekspresji genów", Kierownik projektu - Dr Katarzyna Lisowska |
| Grant Nr Nr 401 1865 33 | (26.11.2007 - 25.11.2010) |
| "Weryfikacja markerów odpowiedzi na radioterapię w raku gruczołu krokowego z zastosowaniem mikrodyssekcji laserowej i metody Q-PCR", Kierownik projektu - Dr Ewa Małusecka |
RESEARCHERS:
- Mieczysław Chorąży, MD, PhD
(+48 32) 278.9842; chorazy@io.gliwice.pl
- Dorota Butkiewicz, PhD
(+48 32) 278.9806; dorotab@rocketmail.com
- Anna Fiszer-Kierzkowska, PhD
(+48 32) 278.9888; anna.fiszer@plusnet.pl
- Katarzyna Lisowska, PhD
(+48 32) 278.9888; kasial@io.gliwice.pl
- Ewa Małusecka,PhD
(+48 32) 278.9647; maluseck@io.gliwice.pl
- Aleksandra Rusin, PhD
(+48 32) 278.9644;aleksandra.rusin@wp.pl
- Marek Rusin, PhD
(+48 32) 278.9806; rusinm@rocketmail.com
- Dorota Ścieglińska, PhD
(+48 32) 278.9679; dorotas@io.gliwice.pl
- Natalia Vydra, PhD
(+48 32) 278.9893; nvydra@yahoo.co.uk
- Wiesława Widłak, PhD
(+48 32) 278.9669; wwidlak@io.gliwice.pl
- Anna Zborek, PhD
(+48 32) 278.9844; azborek@yahoo.com
- Magdalena Głowala-Kosińska, MSci Eng.
(+48 32) 278.9893; mglowala@interia.pl
- Michał Jarząb, MD
(+48 32) 278.9893; mjarzab@io.gliwice.pl
- Małgorzata Krześniak, MSc
(+48 32) 278.9638; gosik1@poczta.fm
- Agnieszka Mazurek, MSc
(+48 32) 278.9679; amazurek@io.gliwice.pl
- Magdalena Olbryt, MSc
(+48 32) 278.9888; molbryt@io.gliwice.pl
- Wojciech Pigłowski, MSc
(+48 32) 278.9679; wpiglowski@o2.pl
FOREIGN FELLOWSHIPS:
(sponsored by NCI-NIH, Bethesda, USA)
- Volga Dudaladava, MSc
Institute of Genetics and Cytology National Academy of Sciences of Belarus
Minsk, Republic of Belarus
- Rasa Vaitiekunaite, MSc
Clinic for Cancer Research and Prevention, Lithuanian Oncology Center Vilnius 2006, Lithuania
- Alexei Mikhailov, MSc
Institute of Biophysics and Cell Engineering National Academy of Sciences of Belarus
Minsk, Republic of Belarus
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